Wang et al. used scRNA-seq analysis to characterize macrophage subtypes related to ischemia-reperfusion-induced AKI and then filtered relevant key genes using cross-sectional bulk RNA-seq data, finding that Ier3 may play an essential role in AKI, with S100a6, Vim, Ier3, and Ccr1 potentially serving as new biomarkers and treatment target for ischemia-reperfusion renal injury (IRI)-induced AKI.64 Here, VIM is linked to acute kidney injury.