ENO2 and infection: Milanes et al. have demonstrated that glucose metabolism is critical for trophozoite viability, providing ATP and key metabolic intermediates, though its precise role in infection remains unclear.9 Their subsequent study identified a single enolase gene (NfENO) in N. fowleri, which shares 47% sequence identity with human enolase (ENO2).10 In humans, ENO2 is a key glycolytic enzyme that converts 2-phosphoglycerate (2-PG) to phosphoenolpyruvate (PEP), a crucial step in energy production, notably upregulated in cancer metabolism.11