The mRNA and protein expression of mitochondria‐related apoptotic factors AIF, P53, Cyt C, APAF‐1, cleaved caspase‐8, BCL‐XL, and NDRG4 were verified using RT‐PCR, WB, and IF, and the results suggested that QSWZZP treated cerebral ischemia primarily by regulating the P53/Cyt C/APAF‐1‐mediated mitochondrial apoptosis pathway. This evidence concerns the gene TP53 and Cerebral ischemia.