To evaluate the prognostic value of the four hub genes (ACSL1, PARP9, TLR4, and ATG3), the GSE31348 gene expression dataset, containing 135 blood samples from 27 pulmonary TB patients assayed at diagnosis and after 1, 2, 4, and 26 weeks of TB treatment, was utilized. The gene discussed is TLR4; the disease is pulmonary tuberculosis.