We hypothesized then that we might have found a local/regional CRYL1 deletion that could be responsible for other unexplained hearing loss cases in our area (northwestern Spain), so we set out to genomically characterize it with the aim of designing a quick and cost-effective PCR assay to screen for it in other deaf patients with heterozygous pathogenic/likely pathogenic GJB2 variants. Here, CRYL1 is linked to hearing loss disorder.