For instance, using single-cell RNA sequencing (scRNA-seq) in RCC to uncover T-cell exhaustion programs co-expressing PD-1, T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), and lymphocyte activation gene 3 (LAG-3) led to trials targeting multiple checkpoints simultaneously. Here, LAG3 is linked to renal cell carcinoma.