Western blot analyses revealed greater GRP78, phopho-IRE1α, phosphor-PERK, and cleaved ATF-6 expression in mice with endotoxemia subjected to VT = 30 mL/kg than in those without endotoxemia subjected to VT = 30 mL/kg and the nonventilated control mice (Figure 2E–H). This evidence concerns the gene ATF6 and serum lipopolysaccharide activity.