The induction of obesity (group 2) led to a significant increase in all monitored inflammatory interleukins compared to the control group (group 1), as observed for IL-1β (Figure 4a), IL-6 (Figure 4b), IL-8 (Figure 4c), TNF-α (Figure 4e), and C-reactive protein (Figure 4f), with p < 0.0001 in all cases. The gene discussed is TNF; the disease is obesity due to melanocortin 4 receptor deficiency.