PCSK1 and esophageal squamous cell carcinoma: Similarly, Zhang and collaborators used Nec-1 to inhibit RIPK1 expression and observed a decrease in cisplatin-induced apoptosis in the human esophageal squamous cell carcinoma cell line KYSE510, and a significant increase in cell proliferation when combining Nec-1 with cisplatin, with respect to monotherapy with the chemotherapy [29].