The nine genes NCAPH, E2F7, NDC80, NCAPG, TOP2A, BUB1B, AURKA, UBE2C, and DLGAP5 are essential in both CRISPR/Cas9 knockout and the RNAi predictability of relative importance, suggesting that the above genes are unique and have potential to be diagnostic biomarkers for PCa cell lines [53]. The gene discussed is TOP2A; the disease is posterior cortical atrophy.