To achieve whether the effects of NR1D2 on cell growth and apoptosis are related to the regulation of the amount and phenotype of CSCs in CRC, we determined ALDH1 + activity, the expression of CD326, CD44 and CD133 cell surface markers and the sphere formation capacity of cells, before and after NR1D2 knock-down. The gene discussed is ALDH1A1; the disease is colorectal carcinoma.