Commonly used research methods in proteomics include TMT proteomics, liquid chromatography–tandem mass spectrometry (LC–MS/MS), Matrix-Assisted Laser Desorption/Ionization Time-of-Flight/Time-of-Flight Mass Spectrometry (MALDI-TOF/TOF), etc. Proteomics helps us to find osteogenic and osteoclastic markers, including Alkaline Phosphatase (ALP), osterix (OSX), the C-terminal telopeptide of type I X-linked collagen (CTX), etc. Meanwhile, the proteomic analysis of plasma, exosomes, and various body fluids allows one to more easily obtain specimens for the diagnosis of osteoporosis. The gene discussed is SP7; the disease is osteoporosis.