In this longitudinal study of 14 patients with recently diagnosed relapsing–remitting multiple sclerosis (RRMS), we evaluated the dynamics of cerebrospinal fluid (CSF) biomarkers, neurofilament light chain (Nf-L), neurofilament heavy chain (Nf-H), and chitinase 3-like protein 1 (CHI3L1) in response to a two-year period of interferon-beta (IFN-β) therapy. Here, NEFH is linked to relapsing-remitting multiple sclerosis.