We also analyzed protein factors released in the supernatants of US-treated cancer cells, detecting well-known tumor markers (e.g., CA19-9, CEA, and CA125) [4], as well as proteins as candidate novel biomarkers for the detection of PC, particularly B2M, CRP, TIMP1, and Galectin-3 [60]. This evidence concerns the gene TIMP1 and pachyonychia congenita.