In order to further verify the potential role of GPX7 in pathophysiology, we examined the expression levels of GPX7 mRNA in the lesion group and the sham-operated group using qRT-PCR (SYBR Green method), and we also examined the expression of IL6 and IL1-beta to demonstrate the success of the cerebral ischemia model construction. This evidence concerns the gene IL6 and brain ischemia.