AKT1 and in situ carcinoma: The total protein amount of AKT remains unaffected by 10 μM and 20 μM CIS treatment in A549 cells (Fig. 5a) and SAS cells (Fig. 5b), while there is a slight decrease in SKOV-3 cells, which is reversed by pre-treatment with 20 μM NIMO (Fig. 5c).