To gain a better understanding of how obesity alters endothelial heterogeneity in WAT, we conducted a high-throughput droplet-based single-cell transcriptomics analysis on viable CD31 + CD45- ECs from vWAT and sWAT under lean and obese conditions (Supplementary Fig. 2 a-c). This evidence concerns the gene PTPRC and obesity due to melanocortin 4 receptor deficiency.