YOD1 and cardiac hypertrophy: To elucidate the deubiquitinating substrate of YOD1 in cardiac hypertrophy, we performed multiple proteomic analyses, including coimmunoprecipitation (co-IP) combined with liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis (interactome), ubiquitinome analysis, and proteome analysis in cardiomyocytes transfected with YOD1 or control plasmid (Fig. 4A).