This study reports quantitative APTw MRI results for simultaneously differentiating ATRX/MGMTp methylation/Ki-67 index/IDH status and p53 expression in WHO grade 4 high-grade glioma (thirty-six IDH wild-type glioblastoma and six IDH-mutant astrocytoma) considering both WHO 2016 and WHO CNS5/2021 classifications. This evidence concerns the gene IDH2 and central nervous system cancer.