In the present study, the non-methylated MGMTp HGG exhibited a significantly higher max APTw% signal vs. methylated tumors in the 42 HGG cases according to WHO 2016 classification including the six IDH-mutated cases, but this finding did not reach significance when re-analyzed to include only the 36 IDHwt glioblastoma patients according to the WHO CNS5/2021 classification (Table S2). This evidence concerns the gene IDH2 and glioblastoma.