Thus, this study aimed to assess the ability of APTw MRI to noninvasively differentiate malignant, high-grade glioma grade 4 as defined by the WHO 2016 and the WHO CNS5/2021 classifications in terms of ATRX expression, MGMTp methylation status, IDH status, Ki-67 index and p53 status. Here, TP53 is linked to central nervous system cancer.