Utilizing our established immune-competent skin model [42], we evaluated the effects of PsA-D on NiSO4-induced activation by analyzing mRNA levels of CD54, CD86, IL-8, IL-6, IL-1β, NLRP3, and COX-2, thereby exploring its potential as a novel therapy for skin sensitization and allergic skin inflammation. Here, NLRP3 is linked to skin sensitization.