Interestingly, 4-OP induced energy metabolism disorder, as evidenced by the decrease in four ATPases (Na+K+-ATPase, Ca2+Mg2+-ATPase, Ca2+-ATPase, and Mg2+-ATPase), the increases in four glycolytic enzymes (HK1, HK2, LDHA, and PGK1), the decrease in glycolytic enzyme PGAM2, and the increase in energy sensor AMPK. Here, HK2 is linked to Other metabolic disease.