Particularly, the expression of transcription factors promoting glial cell differentiation (Olig2, Sox10; Nkx6.2, Nkx2.2, Notch1, etc.)[26,47] was significantly downregulated, as confirmed by transcriptome analysis (Figure 2D), qPCR (Figure 4), and ICC (Figure 7D). This evidence concerns the gene NOTCH1 and intrahepatic cholangiocarcinoma.