DMD and Duchenne muscular dystrophy: To verify dystrophin deficiency in Duchenne muscular dystrophy (DMD) tissue, we conducted an immunohistochemical (IHC) staining assay against dystrophin on muscle sections derived from intercostalis (INT) and pectoralis (PECT) muscle samples of male DMD (n = 6) and wild-type (WT; n = 6) animals.