TNF and infection: In addition, to assess the expression of inflammatory factors during the infection of Ras264.7 macrophages with WT BCG, BCG::ΔmfpA, pMV361-mfpA/BCG::ΔmfpA, and pMV261-mfpA::BCG, we measured the levels of TNF-α and IL-6 in the cell culture supernatants.