AF collected using amniocentesis has been shown to contain a level of MCC as high as 62.4% in cultured samples.45 To demonstrate that AF from vaginal deliveries is a promising source of autologous cells for regenerative medicine and disease modeling, we wanted to ensure that there was no MCC in cultured samples of AFCs collected during a vaginal birth (VB-AFCs). Here, MCC is linked to atrial fibrillation.