TGF‐β, which is used as a positive control, is well‐known for its anti‐inflammatory properties and ability to ameliorate ECM metabolic disorders, exerting beneficial effects on both NPCs and AFCs.[24] First, we performed a CCK‐8 assay to assess the toxicity of β_Man on AFCs and NPCs at different concentrations. The gene discussed is TGFB1; the disease is Other metabolic disease.