Inflammatory cytokine can increase DNA methylation on IL‐1β promoter in chondrocytes, causing remaining inflammation.[29] Decreasing DNA methylation in the promoter regions of certain matrix‐degrading enzymes also enhances the synthesis of these enzymes (such as MMP‐3, MMP‐9, MMP‐13, and ADAMTS‐4), exacerbating the progression of osteoarthritis.[30] We analyzed the expression of methylation‐related genes from the RNA sequencing to investigate the epigenetic mechanism underlying β_Man's regulation of Prkcg expression. The gene discussed is ADAMTS4; the disease is osteoarthritis.