Semen from five infertile men with a total sperm count of more than 20 million sperm (an amount high enough to perform TEM), normal sperm morphology between 1% and 7% and sperm DNA fragmentation, measured by the sperm chromatin dispersion (SCD) assay, of more than 16%, underwent the separation method based on the use of annexin V columns. The gene discussed is ANXA5; the disease is Infertility.