Moreover, hypomethylation in MYO1G promoter (corresponding to high gene expression level) was significantly associated with enhanced infiltration levels of immune cells (CD8+ T cell, M1 macrophage, activated natural killer cells estimated by gene expression), increased cytolytic activity, augmented expression level of immune checkpoint molecules (PDCD1, LAG3, CTLA4, CD274, BTLA, TIGIT, and HAVCR2) and favorable prognosis in melanoma. This evidence concerns the gene MYO1G and melanoma.