In breast cancer, the nontransformed mammary epithelial cell line MCF10A displayed typical mesenchymal characteristics, including reduced E-cadherin and enhanced vimentin, ZEB1, and ZEB2, when following transfection with the P65 subunit of NF-κB or exposure to TNF-α (a potent NF-κB inducer). The gene discussed is CDH1; the disease is breast cancer.