Oral ACN extract significantly enhanced the anti‐mastitis responses in dairy cows and goats, as evidenced by reductions in somatic cell counts (SCC) and decreased expression of pro‐inflammatory cytokines IL‐6, TNFα, and IL‐1β in milk (P < 0.05) (Figures S4 and S5, Supporting Information), inhibited immune cells aggregation, maintained normal distribution of zonula occludens‐1 (ZO‐1) in the mammary tissues and protected the integrity of BMB (Figure 1A–D). This evidence concerns the gene TJP1 and mastitis.