Diagnosis and staging were performed according to Children’s Oncology Group (COG) risk stratification and their clinical features are summarized in Table 1 To characterize and verify primary cultured human neuroblastoma cells (HNB cells) isolated from 8 patient biopsies, we utilized immunohistochemical staining with PHOX2B and synaptophysin markers. The gene discussed is PHOX2B; the disease is neuroblastoma.