In this study, α-MMC was extracted from momordica charantia seeds, and a series of in vitro studies were carried out on lung cancer A549 and 95-D cells, such as cell proliferation, cycle, apoptosis, migration to invasion, etc. Further, Western blot was used to explore the Cyclin-CDK-CKI signaling pathway, Caspase cascade and TNF signaling pathway respectively. This evidence concerns the gene CHKA and lung cancer.