Inbred BTBR and C57BL/6J mice were used to identify genes affecting obesity and glucose metabolism. A ∼316 kb region on chromosome 2 was studied, focusing on Pdk1 and Itga6. Knockout mice lacking Pdk1 or Itga6 were fed an obesogenic diet to assess their role in obesity and glucose tolerance. This evidence concerns the gene ITGA6 and obesity disorder.