Following cell annotation, 13 cell populations were identified, comprising cancer stem cells (n = 2256), liver stem cells (n = 1149), progenitor cells (n = 1976), mucosal cells (n = 1544), CD4+ T cells (n = 6137), CD8+ cytotoxic T cells (n = 3187), regulatory T (Treg) cells (n  = 2540), plasma cells (n  = 1910), naive B cells (n  = 1253), regulatory B cells (n  = 1557), natural killer cells (n  = 1678), macrophages (n  = 7555), and M1 macrophages (n  = 779) (Figure 1B).Figure 1C illustrates the top 10 marker genes of each cell population. Here, CD8A is linked to cancer.