INS and hyperandrogenism: Human PCOS has been modelled in various rat models using hormonal methods, physiological manipulation, and genetical modification, to replicate many phenotypes of human disorder, including hyperandrogenism, LH elevation, polycystic ovaries, and insuline resistance [14].Hormonal methods using androgens and their derivatives are widely used to investigate alterations in endocrine biomarkers and ovarian morphology in PCOS.