To determine whether an SHFV infection induced the formation of SGs via PKR activation, MA104 cells and primary macaque MΦs were infected with SHFV at an MOI of 0.1, and at various times post-infection, the intracellular distribution of G3BP1 was examined by an immunofluorescence assay (IFA). The gene discussed is EIF2AK2; the disease is infection.