Moreover, we checked the expression of MEG3 in a subset of breast cancer cell lines, the results indicated that only the human normal breast cell line MCF10A showed a high expression of MEG3, whereas remarkably decreased expression of MEG3 was observed in all the 7 breast cancer cell lines including MCF7, T47D, SKBR3, MDA-MB-231, DU4475, MDA-MB-453 and HCC1954 (Fig. 1C). This evidence concerns the gene MEG3 and breast cancer.