To investigate the potential role of MEG3 in breast cancer progression, we first established three stable cell lines including MEG3 knockdown cell line using CRISPRi technique in MCF10A, and inducible MEG3 overexpression in two commonly used breast cancer cell lines MDA-MB-231 (TNBC) and MCF7 (Luminal). The gene discussed is MEG3; the disease is breast cancer.