MUC5AC and cystic fibrosis: In this study, we present data on the planktonic growth profiles and biofilm formation of NTHi laboratory and clinical CF isolates in different synthetic sputum media in vitro and present a partially optimized SCFM for usage with NTHi in vitro: SCFM2 with mucin replaced by N-acetylneuraminic acid, supplemented with NAD and haemin (SCFM2.SA.XV).