Our study confirms that 4T1-conditioned media induce the expression of M2 markers such as Arg1, Mrc1 (CD206) and Ccl22. Interestingly, similar to previous studies in a glioblastoma mouse model [69], we have found the upregulation of Cdh1 (Figure 4B), Arg1, Mrc1 (Figure 8C) and F13a1, Hmox1 and Il1r2 (Figure S2) gene expression in macrophages after incubation with 4T1 cell CM. Here, IL1R2 is linked to glioblastoma.