We identified 5 Thai patients diagnosed with Trichophyton indotineae infection after screening of patients with culture results consistent with Trichophyton mentagrophytes complex who had negative urease test and confirmed by molecular analysis in DNA sequencing at the internal transcribed spacer region, including abnormalities in the Squalene epoxidase gene in 2 patients, which confirmed the spread of terbinafine resistant dermatophytosis in Thailand. This evidence concerns the gene SQLE and dermatophytosis.