Case 1, 60 years female with a provisional diagnosis of astrocytoma based on histomorphology and IHC, showed analyzed tumor cells in the sections presented normal copies for both CEP7/EGFR gene, however the ratio of EGFR/CEP7 was >2.0 with an average EGFR gene copy number of >4.0 signals/cells, hence making it positive for EGFR gene mutation (Figure 2a). Here, EGFR is linked to astrocytoma (excluding glioblastoma).