We validated the expression of RNASEK in five cancers, including BLCA, CHOL, LIHC, ESCA, and THCA, via public datasets obtained from the Gene Expression Omnibus (GEO). We employed the GEO2R tool to indicate the differential expression of RNASEK (|Log2FC| > 0.5, adjusted P-value < 0.05), and we utilized the http://www.bioinformatics.com.cn/srplot platform to visualize the volcano plots for differentially expressed genes. This evidence concerns the gene RNASEK and cancer.