Similarly, reverse vaccinology, immunoinformatics and biophysics approaches have been applied to designing of multi-epitopes vaccine against several bacterial viral, parasitic and cancer such as Brugia malayi [99], Escherichia albertii [100], SARS-CoV-2 [101], Acinetobacter baumannii [62], Mycobacterium avium subspecies paratuberculosis [102], Hepatitis C Virus [103], Gardnerella vaginalis [104] and human respiratory syncytial virus (hRSV) [105] and oncogenic KRAS [106]. The gene discussed is KRAS; the disease is cancer.