Plasma membrane rupture caused by bacteria invasion or crystalline aggregates is a known trigger for NLRP3 inflammasome activation in macrophages.[26] Given the observed accumulation of indirubin in the liver of colitis mice, we investigated whether indirubin induces plasma membrane breakdown and NLRP3 inflammasome activation in macrophages in vitro. Here, NLRP3 is linked to colitis.