We only found higher levels of TNF‐α between IBS‐D: 975 ± 95.74 and IBS‐C: 645.36 ± 243.96 (p < 0.05) (Figure 3D) and there were no differences in IL‐10, IL‐4, and IL‐6 between the IBS subtypes (Figure 3A–C). Here, TNF is linked to irritable bowel syndrome.