Epitope spread responses were measured by IFNγ ELISpot after short in vitro stimulation with a mixture of HLA class II-binding epitopes derived from various TAAs expressed in lung cancer, such as NY-ESO-1, KK-LC-1, mesothelin, and mK-RAS, and with HLA class I peptides from hTERT (Figure 5G). The gene discussed is MSLN; the disease is lung carcinoma.