Therefore, this study focused to explore the relationship between CTRP-1, TNF-α, and IL-10 levels, and periodontal inflammation by comparing their levels in GCF samples from systemically healthy individuals with periodontal health, gingivitis, and Stage III Grade B (SIIIGB) periodontitis, as well as to evaluate the effects of NSPT on these biomarkers. The gene discussed is C1QTNF1; the disease is periodontitis.