[64Cu]Cu-NOTA-EV-F(ab’)2 demonstrated significantly higher tumor retention in the H1975 Nectin-4-positive group (5.45 ± 0.57%ID/g) compared to the H520 negative control (1.41 ± 0.09%ID/g, P = 0.0006) and the blocking group (3.10 ± 0.26%ID/g, P = 0.0061). Here, NECTIN4 is linked to neoplasm.