CHRNE and Alzheimer disease: Five of the 6 DMRs were located in the gene body regions of the following genes: ANKylosis Homolog (ANKH), MARS, ANKFY1, Cholinergic Receptor Nicotinic Epsilon Subunit (CHRNE), and KLF2, while the remaining DMR was found in the long non-coding RNA gene, LINC00908. Of these, five DMRs in ANKH, MARS, ANKFY1, CHRNE, and LINC00908 showed lower methylation levels in AD than in CN, whereas one DMR, located in and around KLF2, exhibited a higher methylation level in AD (Fig. 4).