In addition, Wu et al. (2015)31 found that, when comparing mastitis tissue in Holstein cattle to normal tissue, TLR4, MyD88, IL-6, and IL-10 were up-regulated, whereas CD14, TNF-α, MD-2, IL-β, NF-κB, and IL-12 were considerably down-regulated. The gene discussed is NFKB1; the disease is mastitis.